Research Paper Volume 4, Issue 12 pp 952—965

Figure 7. Effect of the studied gero-preventive agents on constitutive level of expression of mTOR-Ser2448^P, RP-S6-Ser235/236^P and 4EBP1-Ser65^P and their corresponding unphosphorylated forms in TK6 cells, detected by western blotting. TK6 cells were exposed to the studied agents at concentrations as shown in Figs 1 and 2 for 4 h. The protein expression level were determined by western blot analysis and the intensity of the specific immunoreactive bands were quantified by densitometry and normalized to actin (loading control). The numbers indicate the n-fold change in expression of the respective phospho-proteins in the drug-treated cultures with respect to the untreated cells (Ctrl).