Research Paper Volume 8, Issue 9 pp 1979—2005

Figure 2. The mir-60 loss dramatically extends lifespan under mild and long-term oxidative stress conditions. (A) Mean adult lifespans of wild-type and mir-60 mutant animals treated with different PQ concentrations are shown. (B) Survival curves of wild-type and mir-60 animals examined under PQ 0 and 5 mM are shown. (C) Distribution of lifespans under the normal condition – the rate of death at each day examined – is shown. (D) The levels of miR-60 examined by qRT-PCR in the transgenics having the mir-60 fragment (mir-60(+/+);mir-60-/-) and the control lines are shown. ‘ud’ denotes undetectable. The levels of another miRNA miR-66, which is known to be expressed constitutively [87], were also tested as a reference. Error bars represent SE calculated from the results of 3 independent trials of sample preparation. (E) Survival curves of the mir-60 transgenics and control lines, which were treated with PQ 5 mM, are shown. The detailed lifespan assay results for (A-C) and (E) are available in Supplemental Figs 1B and 1D, respectively. All lifespan assays shown in this figure were performed at a standard temperature 20 °C. Error bars on the survival curves represent SE calculated from 3-4 replicates. (F) Survival rate of wild-type and mir-60 mutant animals treated with PQ 150 and 200 mM is shown. The assays were performed at 20 °C. Error bars represent SE calculated from 4 replicates. P-values were calculated by unpaired t-test: **p<0.01.