Research Paper Volume 8, Issue 9 pp 2062—2080

Age-associated repression of type 1 inositol 1, 4, 5-triphosphate receptor impairs muscle regeneration

Figure 4. Activation of the EGFR-Ras-ERK pathway in aged muscle. (A) Immunoblot analysis of quadriceps tissue extracts from 4, 12, 20, 27 months mice with anti-ITPR1, anti-phospho EGFR, anti-phospho ERK1/2, and anti-ERK2 antibodies. GAPDH was used as the loading control. n = 2 for each group. (B) Immunoblot analysis of human quadriceps (vastus lateralis) derived from young and aged individuals with anti-ITPR1, anti-phospho EGFR, anti-phospho ERK1/2, and anti-ERK2 antibodies. Actinin was used as the loading control. Young subjects represented individuals less than 60 years of age while aged subjects were greater than 60 years of age. n = 6 for each group.