Research Paper Volume 8, Issue 11 pp 2814—2826

Figure 5. Caloric restriction reduces anxiety of aging mice. (A) An illustration of the Elevated Plus Maze. The maze consists of four arms (two enclosed arms and two open arms) elevated 100 cm above the floor. Anxiety level was determined the time spent in the closed arms (conceived as a safe place) over a 5 minutes testing session. Closed arm duration (in seconds) of (B) Young AL and CR mice, and (C) Old AL and CR mice. (D) The age-dependent changes of anxiety level between AL and CR mice. Significant inverse correlation between closed arm duration and CBF in (E) hippocampus (r² = 0.40, p < 0.0001) and (F) frontal cortex (r² = 0.39, p < 0.0001). Color codes indicate the four groups of mice. Data are mean ± SEM. **p < 0.01; AL: ad libitum; CR: caloric restriction.