Research Paper Volume 8, Issue 11 pp 3028—3044

Rpd3 interacts with insulin signaling in Drosophila longevity extension

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Figure 2. rpd3 reduction affects fly intermediary metabolism. (A-E) Total glucose (A), glycogen (B), trehalose (C), protein (D) and triglyceride (E) levels in rpd3P-UTR/+ (experimental) and rpd3P-1.8/+ (control) male and female flies at age 10 and 40 days. F) Weight of male and female flies used in (C-E). (G). Total triglyceride levels in rpd3P-UTR/+ and rpd3P-1.8 /+ flies aged on 1.5N for 10 or 40 days and then starved for 24 hours. Data are presented as means + SD (n=3, 30 flies per replicate. t test) (H) rpd3 reduction increases stress resistance in flies. Survival curves of rpd3P-UTR/+ and rpd3P-1.8/+ male and female flies during starvation at 10 of age.