Research Paper Volume 8, Issue 11 pp 3028—3044

Rpd3 interacts with insulin signaling in Drosophila longevity extension

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Figure 4. dfoxo partially mediates effects on longevity and stress resistance observed in male rpd3 mutant flies. (A, B) rpd3 (A) and dfoxo (B) mRNA levels in the heads and thoraces of rpd3def/yw, rpd3def/dfoxoc01841and dfoxoc01841/yw male flies at 40 days determined by qPCR (n=3, 30 Heads and thoraces per replicate. p as noted, t test, error bars represent SEM). (C,D) Survival curves of male (C) and female (D) rpd3def/yw, rpd3def/dfoxoc01841 and dfoxoc01841/yw flies. (E-F) Survival curves of rpd3def/+ and control flies male and female flies on 5% H2O2 at age 10 (E) and 40 (F) days. (G) Survival curves of rpd3P-UTR/+ and rpd3P-1.8/+ male and female flies exposed to 5% H2O2 at age 10 days of age. (H, K) Survival curves for male (H, J) and female (I, K) rpd3def/yw, rpd3def/dfoxoc01841 and dfoxoc01841/yw flies during starvation (H, I) or on 5% H2O2 (J, K) at age 40.