Research Paper Volume 10, Issue 2 pp 197—211

The role of autophagy during murine primordial follicle assembly

Figure 7. Rapamycin-promoted autophagy decreased the level of apoptosis. (A) TUNEL histochemistry (red) in tissue sections of ovaries from control, rapamycin and 3-MA treated ovaries for 3 days. (B) Autophagy decreased the number of TUNEL positive cells. (C) Quantitative RT-PCR for Bax/Bcl-2 mRNA levels in control, rapamycin and 3-MA treated ovaries for 3 days. (D) WB analysis of BAX/BCL-2 in control, rapamycin and 3-MA treated ovaries for 3 days. The results are presented as mean ± SD. *P < 0.05; **P < 0.01.