Research Paper Volume 10, Issue 2 pp 197—211

The role of autophagy during murine primordial follicle assembly

Figure 8. Sirt1 inhibition depressed autophagy and caused an over loss of germ cells. (A) Level of SIRT1 protein in control, EX527 and Sirt1 RNAi treatment for 2 days ovaries. (B) Level of H4K16ac protein in control, EX527 and Sirt1 RNAi treatment for 2 days ovaries. (C) WB analysis of LC3II/LC3I in control, EX527 and Sirt1 RNAi treated ovaries (6 h). (D) WB analysis of BAX/BCL-2 in control, EX527 and Sirt1 RNAi treated ovaries for 3 days. (E) IF staining for MVH (red) of control, EX527 and Sirt1 RNAi treated mouse ovaries for 3 days and 5 days. (F) Percentage of germ cells in cysts and follicles in the three groups after 3 days treatment. (G) Average number of survived oocytes in the three groups after 3 days treatment. (H) Percentage of germ cells in cysts and follicles in the three groups after 5 days treatment. (I) Average number of survived oocytes in the three groups after 5 days treatment. The results are presented as mean ± SD. * P < 0.05; ** P < 0.01.