Research Paper Volume 10, Issue 4 pp 632—644

KCNQ1OT1 promotes melanoma growth and metastasis

Figure 3. miR-153 can target MET. (A) Correlation between MET and miR-153 expression. (B) Prediction that miR-153 can specifically bind to the MET 3′ UTR by MIRDB. (C, D) Fluorescence intensity was measured in HACAT cells co transfected with miR-153 or miR-153 as and MET WT/mut construct. Vector was used as a control. (E, F) After transfecting A375 cells with miR-153 mimic, the MET protein and mRNA levels were detected by western blot and real time PCR. Data are shown as mean ± SEM. ** P< 0.01 (G, H) After transfecting A375 cells with miR-153 AS, MET protein and mRNA levels of was detected by western blot and real-time PCR. Data are shown as mean ± SEM. ** P< 0.01.