Research Paper Volume 10, Issue 4 pp 632—644

KCNQ1OT1 promotes melanoma growth and metastasis

Figure 5. KCNQ1OT1 promotes melanoma cell proliferation. (A) After transfecting cells with KCNQ1OT1, proliferation rates of A375 and A875 were determined using MTT assays. Data are presented as means ± SEM. (B) After transfecting cells with si-KCNQ1OT1, the proliferation rates of A375 and A875 were determined using MTT assays. Data are presented as means ± SEM. (C, D) After transfecting KCNQ1OT1/control in A375 cells, the cyclin d1 protein and mRNA level was detected by western blot and real time PCR. Data are shown as mean ± SEM. ** P< 0.01 (E, F) After transfecting si-KCNQ1OT1 or control in A375 cells, cyclin d1 protein and mRNA level was detected by western blot and real time PCR. Data are shown as mean ± SEM. ** P< 0.01.