Research Paper Volume 10, Issue 4 pp 632—644

KCNQ1OT1 promotes melanoma growth and metastasis

Figure 6. KCNQ1OT1 promotes melanoma cell metastasis. (A, B) After transfecting KCNQ1OT1/control in A375 cells and (E, F) A875 cells, transwell assays with or without matrigel were performed. Cells were counted and results represent the mean ± SD of three experiments. ** P < 0.01 (C, D) After transfecting si-KCNQ1OT1 or control in A375 cells and (G, H) A875 cells, transwell assays with or without matrigel were performed. Cells were counted and results represent the mean ± SD of three experiments. ** P < 0.01 (I, J) After transfecting KCNQ1OT1/control or (K, L) si-KCNQ1OT1 in A375 cells, MMP2 protein and mRNA level was detected by western blot and real time PCR. Data are shown as mean ± SEM. ** P < 0.01 (M) 2×106 A375 cells with stable expression of vector (control), KCNQ1OT1, and si-KCNQ1OT1 were injected into nude mice via the tail vein. Livers were dissected and either macroscopically photographed (left) or stained with H&E (middle, Scale bar, 100μm). The right side is the HE staining results of the liver (right).