Research Paper Volume 10, Issue 4 pp 632—644

KCNQ1OT1 promotes melanoma growth and metastasis

Figure 7. miR-153 mediates the effect of KCNQ1OT1 on proliferation and metastasis in melanoma cells. (A) After transfecting with control, miR-153, or KCNQ1OT1 and miR-153, A375 and A875 cell proliferation was detected using an MTT assay. Data are shown as mean ± SEM. (B) After transfecting control, miR-153, or KCNQ1OT1 and miR-153 in A375 cells, a transwell assay without matrigel was performed. Cells were counted and results represent the mean ± SD of three experiments. ** P < 0.01 (C, D) Western blot and real time PCR was used to detect indicant proteins in A375 cells. ** P < 0.01 (E) After transfecting with control, AS-miR-153, or si-KCNQ1OT1 and miR-153 AS, A375 and A875 cell proliferation was detected using an MTT assay. Data are shown as mean ± SEM. (F) After transfecting control, ASO-miR-153, or si-KCNQ1OT1 and miR-153 AS in A375 cells, a transwell assay without matrigel was performed. Cells were counted and results represent the mean ± SD of three experiments. ** P< 0.01 (G, H) Western blot and real time PCR were used to detect indicant proteins in A375 cells. ** P< 0.01.