Research Paper Volume 10, Issue 4 pp 747—763

Oxidative stress-mediated senescence in mesenchymal progenitor cells causes the loss of their fibro/adipogenic potential and abrogates myoblast fusion

Figure 4. Adipogenic differentiation ability was decreased in PMS-2G11 cells. (A) Immunocytochemical analysis of PPARγ in 2G11 and PMS-2G11 cells treated with or without bFGF. Arrowheads: PPARγ+ cells. Scale bar: 100 μm. (B) Quantification of PPARγ+ cells. Data are expressed as means±SE (n=3); ***P<0.001. (C) Immunocytochemical analysis of perilipin in 2G11 and PMS-2G11 cells treated with or without bFGF. Arrowheads: perilipin+ cells. Scale bar: 100 μm. (D) Quantification of perilipin+ areas. Distinct letters (a, b) indicate statistically significant differences (P<0.05).