Research Paper Volume 10, Issue 6 pp 1281—1293

CircSNCA downregulation by pramipexole treatment mediates cell apoptosis and autophagy in Parkinson’s disease by targeting miR-7

Figure 2. The expression of SNCA and apoptotic-related genes in MMP+ treated SH-SY5Y cells with or without PPX treatment. (A) Cell viability of MMP+ treated SH-SY5Y cells increased with the increase of PPX concentration. *P < 0.05 compared with NC (nothing control), #P < 0.05 compared with PD-model (MPP+ 2.5 mM). (B) The relative mRNA expression detected by qRT-PCR of SNCA and apoptosis-related genes (BCL2, CASP3, BAX, PTEN and P53). PPX treatment partly offset the influence of MMP+ on the expression of these mRNAs. *P < 0.05 compared with NC (nothing control), #P < 0.05 compared with PD-model (2.5 mM). (C) The protein expression of SNCA and apoptosis-related genes (BCL2, CASP3, BAX, PTEN and P53) detected by western blot. PPX treatment partly offset the influence of MMP+ on the expression of these proteins. *P < 0.05 compared with NC, #P < 0.05 compared with PD-model (MPP+ 2.5 mM).