Research Paper Volume 10, Issue 6 pp 1281—1293

CircSNCA downregulation by pramipexole treatment mediates cell apoptosis and autophagy in Parkinson’s disease by targeting miR-7

Figure 4. The target relationship among circSNCA, miR-7 and SNCA mRNA of PD. (A) (a) 3’-UTR region of SNCA mRNA and hsa_circ_0127305 were both found to harbor a binding site for miR-7. (b) Luciferase reporter assay results showed that miR-7 exclusively reduced the luciferase activity of the wild-type reporter plasmids of circSNCA. (c) Luciferase reporter assay results showed that miR-7 exclusively reduced luciferase activity of the wild-type reporter plasmids of circSNCA. (B) RNA FISH for co-localization of circSNCA and miR-7 in cytoplasm of SH-SY5Ys. CircSNCA and miR-7 probes were labeled with Alexa 488 and Cy-5, respectively. Nuclei were stained with DAPI. Scale bar = 10 μm.