Figure 6. Dose-dependent decrease or increase of the number of meiotic germ cells (RAD51) and of γH2AX positive cell, respectively, in nicotine treated fetal ovaries cultured for 4 days. (A) Representative IF images of ovarian tissue sections for RAD51 and γH2AX; (B) Relative percentage of RAD51 positive cells of ovaries cultured without (control) and with 1mM or 10mM nicotine. (C) WB and relative densitometric analyses of DAZL amount in control and nicotine exposed ovaries. (D) Representative WB and relative densitometric analyses of RAD51 amount in control and nicotine exposed ovaries. All experiments were repeated at least three times. Results are presented as mean ± SD. (*) and (**) indicate significant (P < 0.05) and highly significant (P < 0.01) difference, respectively.