Research Paper Volume 10, Issue 7 pp 1556—1574

Nicotine exposure impairs germ cell development in human fetal ovaries cultured in vitro

Figure 8. Dose-dependent nicotine decrease of antioxidative enzyme gene transcripts in fetal ovaries cultured for 4 days. (A) qRT-PCR for Sod1, Cat and Gpx mRNA levels in control and nicotine treated ovaries. The expression levels were normalized to that of Gapdh gene. (B) Representative WB and relative densitometric analyses of SOD1 proteins in control and nicotine treated ovaries. (C) WB and relative densitometric analyses of CAT amount in control and nicotine exposed ovaries. (D) The results of p38MAPK WB and relative densitometric analyses in control and nicotine exposed ovaries. All experiments were repeated at least three times. Results are presented as mean ± SD. (*) and (**) indicate significant (P < 0.05) and highly significant (P < 0.01) difference, respectively.