Research Paper Volume 10, Issue 7 pp 1666—1681

Mitochondria-targeted hydrogen sulfide attenuates endothelial senescence by selective induction of splicing factors HNRNPD and SRSF2

Figure 2. Cell proliferation rate is not affected by H2S donor treatment. (A) Proliferation index was assessed for treated cells as assessed by Ki67 immunofluorescence (>400 nuclei counted per sample). (B) Cell counts following 24h treatment with Na-GYY4137 at 100 µg/ml, AP39, AP123, RT01 at 10 ng/ml. (C) Apoptotic index in senescent cells treated with inhibitors as determined by TUNEL assay. Data are derived from duplicate testing of 3 biological replicates. (D) Telomere length was assessed by qPCR in three biological and 3 technical replicates. (E) BrdU incorporation into cellular DNA. Relative BrdU incorporation was assessed in 3 biological replicates and was calculated by normalization of data to values corresponding to untreated (control) cells and are expressed as % BrdU incorporation. (F) Effect of 24h treatment with Na-GYY4137 at 100 µg/ml, AP39, AP123, RT01 at 10 ng/ml on accumulation of senescent cells over 2 passages in early passage cells (PD = 44). Mean+- SD of three independent experiment is shown. Statistical significance is indicated by *** p<0.001. Error bars represent the standard error of the mean.