Figure 4. Effects of culture with different concentrations of sTNF-α or sFasL on activation susceptibility, embryo developmental potential and cytoplasmic fragmentation of aging DOs. While (A) and (B) show rates of ethanol-activated oocytes, (C) and (D) show blastocyst rates of Sr2+ activated oocytes. Mouse DOs collected at 13 h post hCG were cultured for 12 h in CZB medium containing different concentrations of sTNF-α or sFasL before activation treatment with ethanol or SrCl2. Each treatment was repeated 3 times with each replicate including about 30 oocytes. E and F show percentages of fragmented oocytes after newly ovulated DOs were cultured for 36 or 48 h in CZB with different concentrations of sTNF-α (E) or sFasL (F). Each treatment was repeated 4 times with each replicate containing about 30 oocytes. a–c: Values with a different letter above bars differ significantly (P < 0.05).