Research Paper Volume 10, Issue 10 pp 2723—2740

Long-term intake of Lactobacillus paracasei KW3110 prevents age-related chronic inflammation and retinal cell loss in physiologically aged mice

Figure 5. Protective effect of Lactobacillus paracasei KW3110 on age-induced histological changes and ganglion cell loss in the retina. (A) Hematoxylin and eosin staining of retinal sections in young mouse (3-months-old) fed a control (CTL) diet and aged mice (22-months-old) fed a diet either with or without L. paracasei KW3110 (KW3110 diet). Arrow heads indicate the ganglion cell layer (GCL) and outer nuclear layer (ONL), respectively. Scale bar represents 100 μm. (B) The survival rate of retinal ganglion cells (RGCs) in aged mice (22-months-old) fed a diet either with or without L. paracasei KW3110 (KW3110 diet) were analyzed compared to the survival of RGCs in young mice (3-months-old) fed a control diet. Values are presented as the means ± SEM. Significance was assumed if the p value was < 0.05. **p < 0.01. (C) ONL thickness was lower in aged mice (22-months-old) fed control diet than in aged mice fed a diet with L. paracasei KW3110. Values are presented as the means ± SEM. Significance was assumed if the p value was < 0.05. *p < 0.05; **p < 0.01.