Research Paper Volume 10, Issue 11 pp 3117—3135

Transferrin is responsible for mediating the effects of iron ions on the regulation of anterior pharynx-defective-1α/β and Presenilin 1 expression via PGE2 and PGD2 at the early stage of Alzheimer’s Disease

Figure 3. Involvement of COX-2 in mediating Fe-induced expression of APH-1α/1β and PS1 in neurons. (A, B) APP/PS1 Tg mice were injected (i.c.v) with M-30 (1 μg/5 μl) or NS398 (1 μg/5 μl) for 48 h. (C, D) In select experiments, C57BL/6 mice were injected (i.c.v) with Fe (1 μg/5 μl) in the absence or presence of M-30 (1 μg/5 μl) or NS398 (1 μg/5 μl) for 48 h. (E, F) In separate experiments, n2a cells were treated with Fe (10 μM) in the absence or presence of M-30 (10 μM) or NS398 (10 μM) for 48 h. The mRNA and protein levels of APH-1α/1β and PS1 were determined by qRT-PCR and western blots, respectively. GAPDH and β-actin served as internal controls. The data represent the means±S.E. of all the experiments. *p<0.05; **p<0.01 compared with C57BL/6 or n2a controls. # p<0.05 with respect to APP/PS1 or Fe-treated mice alone.