Research Paper Volume 10, Issue 11 pp 3148—3160

BK channel overexpression on plasma membrane of fibroblasts from Hutchinson-Gilford progeria syndrome

Figure 1. Outward currents patch-clamp recorded in whole-cell configuration. (A) Representative examples of current traces recorded in hDF obtained from a young donor, an elderly, and a patient affected by HGPS. Current traces recorded after 100 nM IbTx application and a graphical representation of the pulse protocol (holding potential at 0 mV) are also shown. (B) Average ± SEM of current-voltage relationships (I–V) recorded in hDF obtained from healthy donors (Young, n=83; Elderly, n=16) and patients affected by HGPS (n=80). (C) Average ± SEM of current-voltage relationships (I–V) recorded in hDF obtained from young donors and patients affected by HGPS treated by 100 nM IbTx (n=6) and 10 mM TEA (n=4). Young vs. HGPS: *p<0.05; **p<0.01; Young vs. Elderly: #p<0.05; ##p<0.001; ###p<0.0001.