Figure 1. PrC with high SPC25 expression is associated with poor overall survival. (A-B) RT-qPCR for SPC25 mRNA levels in 40 PrC specimens and paired normal prostate tissue (NT), shown by mean ± SD (A), and by individual values (B). (C-D) ELISA for SPC25 protein levels in 40 PrC specimens and NT, shown by mean ± SD (C), and by individual values (D). (E) Immunostaining for SPC25 (fast red) in PrC and NT. (F) The 40 patients were followed-up for 5 years. The patients were classified into SPC25-high group (n=20) and SPC25-low group (n=20), based on the median value. Kaplan-Meier curves were plotted. *p<0.05. N=40. Scale bars are 100 µm.