Research Paper Volume 10, Issue 11 pp 3273—3282

Spindle pole body component 25 regulates stemness of prostate cancer cells

Figure 2. SPC25 promotes PrC cell growth and migration. Two human PrC cell lines (DU145 and LNCap) were used in in vitro studies. (A) RT-qPCR for SPC25 in DU145 and LNCap cells. (B) LNCap cells were transfected with a SPC25-expressing plasmid, and RT-qPCR for SPC25 was done on transfected cells, compared to control. (C) DU145 cells were transfected with a plasmid carrying short hairpin small interfering RNA for SPC25 (shSPC25), and RT-qPCR for SPC25 was done on transfected cells, compared to control. (D-E) MTT assay for transfected LNCap cells (D), and transfected DU145 cells (E). (F-G) Transwell cell migration assay for transfected LNCap cells (F), and transfected DU145 cells (G). *p<0.05. N=5. Scale bars are 20 µm.