Research Paper Volume 10, Issue 11 pp 3273—3282

Spindle pole body component 25 regulates stemness of prostate cancer cells

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Figure 2. SPC25 promotes PrC cell growth and migration. Two human PrC cell lines (DU145 and LNCap) were used in in vitro studies. (A) RT-qPCR for SPC25 in DU145 and LNCap cells. (B) LNCap cells were transfected with a SPC25-expressing plasmid, and RT-qPCR for SPC25 was done on transfected cells, compared to control. (C) DU145 cells were transfected with a plasmid carrying short hairpin small interfering RNA for SPC25 (shSPC25), and RT-qPCR for SPC25 was done on transfected cells, compared to control. (D-E) MTT assay for transfected LNCap cells (D), and transfected DU145 cells (E). (F-G) Transwell cell migration assay for transfected LNCap cells (F), and transfected DU145 cells (G). *p<0.05. N=5. Scale bars are 20 µm.