Research Paper Volume 10, Issue 11 pp 3327—3352

Figure 5. Upon aging, the tibialis anterior and soleus undergo similar changes in mitochondrial biogenesis, fission/fusion, disposal and autophagy marker expression. Markers of mitochondrial biogenesis, fission/fusion, disposal and autophagy were evaluated in tibialis anterior (A, C) and soleus (B, D) muscles isolated from young (3 mo) and old (29 mo) mice. A-B) Representative OXPHOS subunits, PGC-1α, PGC-1β, Mfn2, short (S)- and long (L)-OPA1, LC3-II/I, and APG5 in whole muscle lysates were evaluated by Western blotting and normalized to Ponceau-stained total protein (see Figures S6, S7). The mean plus standard deviation of 3 technical replicates of lysates from 4-5 mice/group is shown; 3 mo (n=5), 29 mo (n=4). C-D) Mfn2, OPA1, and p62 levels in mitochondrial fractions were evaluated by Western blotting and normalized to Ponceau-stained total protein (see Figure S7). The mean plus standard deviation of lysates from 4 mice/group run on 2 separate gels is shown. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.