Research Paper Volume 11, Issue 1 pp 63—72

Mesenchymal stem cells rejuvenate cardiac muscle after ischemic injury

Figure 4. Enhanced cell proliferation by CD146+MSCs against hypoxia in cardiac muscle cells. (A) A transwell system was used to co-culture primary CMCs with different MSCs under hypoxia condition for 24 hours. Four conditions were applied. CMCs were plated at the lower plate. Condition 1, upper plate contained control media in normoxia condition (CTL). Condition 2, upper plate contained control media in hypoxia condition (Hypoxia). Condition 3, the upper plate contained same number of tMSCs (to the number of CMCs in lower plate) in hypoxia condition (Hypoxia+tMSCs). Condition 4, the upper plate contained same number of CD164+MSCs (to the number of CMCs in lower plate) in hypoxia condition (Hypoxia+CD164+MSCs). (B-C) BrdU+ CMCs were analyzed, shown by representative flow charts (B), and by quantification (C). *p<0.05. N=5.