Research Paper Volume 11, Issue 1 pp 127—159

Premature aging and cancer development in transgenic mice lacking functional CYLD

Figure 9. Alterations found in the pancreas, liver, lung and stomach of the K5-CYLDC/S mice suggestive of early aging of the K5-CYLDC/S mice. (A-H) Histopathologic analysis by H&E staining of pancreas from 5-month-old (B, F) and 12-month-old Control and transgenic mice. (A, E, F) Pancreas from Control mice: note the presence of Islets of Langerhans (L) of heterogeneous but moderate size. (B-D; G, H) Histology sections representatives of pancreas from K5-CYLDC/S mice. Note the hyperplasia of the Islets of Langerhans (B, C, G, H). (D) Extrapancreatic location of the Islets of Langerhans, in the peripancreatic fat, observed in the K5-CYLDC/S mice. (H) Foci of inflammation (asterisk) in the pancreas of K5-CYLDC/S mice. (I-N) Histopathological analysis of liver, lung and stomach sections from different organs of 20-month-old K5-CYLDC/S mice. (I-K) Representative images showing anisokariosis (I), eosinophilic intracytoplasmic inclusions (arrow in J), intranuclear eosinophilic inclusions (arrow in K), and inflammation foci (asterisks in L) in the liver. (M) Example of inflammation foci observed in the lung. (N) Stomach with an inflammation focus. The pancreas of 4 Control and 4 transgenic mice of 5- and 12-month-old were analyzed. Number of animals whose liver, lung and stomach has been analyzed is showed in Table 1. Asterisks: Inflammation. Scale bars: 250 μm (A, B, D-F); 350 μm (C); 500 μm (G); 150 μm (H; L-N); 40 μm (I-K).