Figure 8. The combination of BCL2L10 and Beclin 1 reduced the bonding of Beclin 1 and PI3KC3 in Hep3B cell line. (A) The activity of PI3KC3 after the overexpression of BCL2L10 was detected by ELISA; (B) The activity of PI3KC3 after inhibiting BCL2L10 was detected by ELISA; (C) The binding relation between PI3KC3 and BECN1 was detected by co-immunoprecipitation. (D) Bioinformatics turned out that PI3K/Akt signaling pathway was enabled in HCC. (E) PI3K p110α and p-AKT protein level was detected by Western blot after autophagy induction. (F) The BECN1 protein level was detected by western blot after adding PI3K inhibitor and AKT inhibitor. * P<0.05 ** P<0.01 compared with CM/NC group, # P<0.05 compared with EBSS/NC group.