Figure 6. The effect of 3,4,5-tricaffeoylquinic acid (TCQA) on cell cycle and phosphorylation of tumor protein p53 in human neural stem cells (hNSCs). Cell cycle was determined by labeling cellular DNA. hNSCs were treated with growth medium or differentiation medium with or without 10 μM TCQA for 24 h. (A) Ratio of each cell cycle phase as a percentage of total cells. (B) The histograms show the cells in G0/G1 (pink peak on left), S (green center peak) and G2/M (blue peak on right). (C) hNSCs were treated with growth medium or differentiation medium with or without 10 μM TCQA for 24 - 96 h. Data was set as % of undifferentiated control. Data were presented as mean ± SD. * P < 0.05, ** P < 0.01 Compared with undifferentiated control cells. # P < 0.05 significance by student’s t test.