Figure 3. Effect of FH+/– KO on proliferation, apoptosis and cell cycle. All experiments were performed on the cells at approximately 25 passages. (A) and (B) The growth curves and images, respectively, generated by the Incucyte Zoom system. A representative flow cytometry cell cycle analysis is shown in (C), and the corresponding histogram of the cell cycle analysis is shown in (D), where the bar graphs represent the percentage of WT and FH+/– cells in the G2/M, S and G0/G1 phases. Early apoptosis detected by flow cytometry is shown in (E), and the corresponding histogram is shown in (F), where the bar graphs represent the percentage of apoptotic cells.