Research Paper Volume 11, Issue 2 pp 615—633

MiR-223-3p promotes cell proliferation and metastasis by downregulating SLC4A4 in clear cell renal cell carcinoma

Figure 3. Bioinformatic analysis of miR-223-3p target genes in ccRCC. (A) Bioinformatic prediction of the top 20 mRNA targets of miR-223-3p in TargetScan and miRDB. (B) Heat map depicting the expression of miR-223-3p and five target genes in samples from TCGA-KIRC. (C) Correlation analysis of the expression of miR-223-3p and five target genes in cancer samples from TCGA-KIRC. (D) Relative mRNA expression of five target genes in cancer samples from TCGA-KIRC. (E) A qRT-PCR analysis demonstrated the negative correlation between SLC4A4 and miR-223-3p expression in ccRCC tissues (R = -0.437, p = 0.037). Data are shown as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.