Research Paper Volume 11, Issue 2 pp 615—633

MiR-223-3p promotes cell proliferation and metastasis by downregulating SLC4A4 in clear cell renal cell carcinoma

Figure 4. SLC4A4 is a direct target of miR-223-3p. (A) Western blotting and (B) qRT-PCR analysis of SLC4A4 expression in 786-O and Caki-1 cells transfected with miR-223-3p mimics versus the corresponding NC. (C) Western blotting and (D) qRT-PCR analysis of SLC4A4 expression in 786-O and Caki-1 cells transfected with miR-223-3p inhibitors versus the corresponding NC. (E) The predicted binding sites for miR-223-3p in the SLC4A4 3′-UTR. The red nucleotides are the seed-pairing target sites of miR-223-3p. (F) Luciferase reporter assays demonstrate that the reporter activity of 786-O and Caki-1 cells decreased by approximately 50% upon co-transfection of the wild-type SLC4A4 3′-UTR reporter construct and miR-223-3p mimics. Data are shown as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.