Research Paper Volume 11, Issue 2 pp 615—633

MiR-223-3p promotes cell proliferation and metastasis by downregulating SLC4A4 in clear cell renal cell carcinoma

Figure 6. SLC4A4 overexpression inhibits cell proliferation, migration and invasion in vitro. SLC4A4 was downregulated in ccRCC tissues, as shown by (A) qRT-PCR and (B) immunohistochemistry analysis. (C) SLC4A4 was downregulated in ccRCC tissues, as shown by immunohistochemistry analysis from the Human Protein Atlas. (D) Gene set enrichment analysis comparing low and high SLC4A4 expression groups in the database of TCGA. Enrichment curves are shown for activated gene sets related to the epithelial-mesenchymal transition and KRAS signaling. (E) and (F) SLC4A4 mRNA and protein levels were successfully upregulated in 786-O and Caki-1 cells, and SLC4A4 overexpression inhibited KRAS expression. (GI) SLC4A4 overexpression inhibited (G) proliferation, (H) migration and (I) invasion in Caki-1 cells. Data are shown as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001.