Research Paper Volume 11, Issue 4 pp 1089—1109

MALAT1/miR-15b-5p/MAPK1 mediates endothelial progenitor cells autophagy and affects coronary atherosclerotic heart disease via mTOR signaling pathway

Figure 5. VCAM-1/ICAM-1 expression and mTOR signaling pathway expression in cells. (A) QRT-PCR results exhibited that sh-MALAT1#1, sh-MALAT1#2 and CCCP suppressed VCAM-1 and ICAM-1 expression and there was conspicuous difference between normal group and NC group, knockdown of MALAT1 could inhibit CAD progress effectively. *P<0.05, **P<0.01, compared with NC group; ##P<0.01, compared with normal group. (B) Transmission electron microscope showed the autophagosome in sh-MALAT1#1 or, sh-MALAT1#2 and CCCP group was more than in NC group. (C) mTOR signaling pathway expression in different transfection groups. *P<0.05, **P<0.01, compared with NC group; ##P<0.01, compared with normal group. (D) ATG1 and LC3-II expression level in different transfection groups. P<0.05, **P<0.01, compared with NC group; ##P<0.01, compared with normal group.