Research Paper Volume 11, Issue 4 pp 1089—1109

MALAT1/miR-15b-5p/MAPK1 mediates endothelial progenitor cells autophagy and affects coronary atherosclerotic heart disease via mTOR signaling pathway

Figure 8. LncRNA MALAT1 enhanced CAD progression via activating mTOR signaling pathway. (A, B) QRT-PCR results of VCAM-1/ICAM-1 expression in different transcription groups. **P<0.01, compared with NC group. (C, D) Western blot results showed that both sh-MALAT1#1 and miR-15b-5p mimics depressed expression of ERK1/2, phosphorylated mTOR. Both CCCP and miR-15b-5p mimics promoted expression of ATG1 and LC3-II. Nevertheless, both miR-15b-5p inhibitor and pCMV6-MAPK1 suppressed expression of ATG1 and LC3-II. Nevertheless, both miR-15b-5p inhibitor and pCMV6-MAPK1 enhanced expression of MPKA1 and phosphorylated mTOR. MiR-15b-5p inhibitor + sh-MALAT1#1 group, miR-15b-5p mimics + pCMV6-MALAT1 as well as miR-15b-5p mimics + pCMV6-MAPK1 group exhibited similar results of NC group. *P<0.05, **P<0.01, compared with NC group.