Figure 3. Suppressing ERK5 activation prevents HG-induced M2a polarization of microglia and induces earlier polarization of M2b-like followed by M1-like microglia. (A) BIX021895 (BIX) was applied to the HG-cultured microglia. (B) Western blot for pERK5, compared to the total ERK5 levels in HG- cultured microglia at different time courses, with or without BIX. (C–I) RT-qPCR for iNOS (C), TNF-α (D), IL-6 (E), IL-12 (F), IL-10 (G), Arg-1 (H) and CD206 (I) mRNA levels in HG- cultured microglia at different time points. (J) TUNEL assay on HCN-2 cells. N=5. *p<0.05.