Research Paper Volume 11, Issue 2 pp 707—723

Age-related immune-modulating properties of seminal fluid that control the severity of asthma are gender specific

Figure 3. Human seminal fluid does not improve pathological changes in asthmatic male mice. (A) Schematic representation of experimental design for human seminal fluid (hSF) exposure. Young adult male mice sensitized with ovalbumin (OVA) were given hSF intraperitoneally 30 min before OVA challenge. (B) Changes in Th2-cell-driven allergic responses in asthmatic male mice exposed to hSF. White box: control group (n = 3); colored boxes: asthma groups (n = 5–7). Data are presented as means ± SEM. *P < 0.05 versus OVA asthma group. (C) Representative images of PAS staining of lungs of asthmatic male mice exposed to hSF. AW: airway. (D) Transcriptional induction of osteopontin and IL-17A in lungs of asthmatic male mice exposed to hSF. White box: control group (n = 3); colored boxes: asthma groups (n = 5 - 7). Data are presented as means ± SEM. *P < 0.05 versus OVA asthma group. (E) Transcriptional induction of osteopontin by hSF in antigen-stimulated BM-DCs of 2-month-old male mice. White box: control group (n = 3); colored boxes: OVA-stimulated groups (n = 6 each). Data are presented as means ± SEM. *P < 0.05 versus OVA group.