Research Paper Volume 11, Issue 3 pp 898—907

p38MAPK/SGK1 signaling regulates macrophage polarization in experimental autoimmune encephalomyelitis

Figure 4. Generation of an adeno-associated virus (AAV) carrying sh-p38 or sh-SGK1 under the control of a CD68 promoter. An adeno-associated virus (AAV) carrying sh-p38 or sh-SGK1 under the control of a CD68 promoter (AAV-sh-p38, AAV-sh-SGK1) was generated to specifically knockdown p38 or SGK1 exclusively in macrophages. (A-B) RT-qPCR for p38 (A) or SGK1 (B) levels in AAV-sh-p38 or AAV-sh-SGK1-treated macrophages in vitro. (C-E) mRNA levels of Arg-1 (C), Ym-1 (D) and Fizz-1 (E) by RT-qPCR. (F) Arginase assay. (G) Flow cytometry for CD206 in control (CTL), sh-p38, or sh-SGK1-treated macrophages that were exposed to IL-4. *p<0.05. NS: non-significant. N=5.