Figure 1. MiR-449 is a PLOD1-targetting miRNA low expressed in ASCs. (A) PLOD1 protein levels were examined in rat and human ASCs by Western blotting. Rat2 and HSFs cells treated with/without TGFβ1 were used as controls. (B) Bioinformatics tools were used to find 3 PLOD1-targeting miRNAs (miR-34, miR-140 and miR-449) conserved in rat and human. (C) RT-qPCR for the 3 PLOD1-targeting miRNAs (miR-34, miR-140 and miR-449) in rat and human ASCs. (D-E) The binding sites of miR-449 on human (D) and rat (E) PLOD1 3’-UTR were shown. *p<0.05. NS: non-significant. N=5.