Research Paper Volume 11, Issue 5 pp 1333—1341

The long non-coding RNA PTTG3P promotes growth and metastasis of cervical cancer through PTTG1

Figure 2. PTTG3P is upstream of PTTG1 in CC. (A-B) We overexpressed PTTG1 or depleted PTTG1 (by expressing short hairpin interfering RNA for PTTG1-shPTTG1) in two CC lines, Ca-Ski and HTB31. Plasmids carrying a scrambled sequence (SCR) were used as controls. (A) RT-qPCR for PTTG1. (B) RT-qPCR for PTTG3P. (C-D) We overexpressed PTTG3P or depleted PTTG3P (by expressing short hairpin interfering RNA for PTTG3P-shPTTG3P) in two CC lines, Ca-Ski and HTB31. (C) RT-qPCR for PTTG3P. (D) RT-qPCR for PTTG1. *p<0.05 (versus SCR). NS: non-significant. N=5.