Research Paper Volume 11, Issue 5 pp 1427—1439

FoxD2-AS1 promotes glioma progression by regulating miR-185-5P/HMGA2 axis and PI3K/AKT signaling pathway

Figure 2. LncRNA FOXD2-AS1 acts as a sponge of miR-185-5p. (A) The wild type (wt) and mutant (mut) binding sites of miR-185-5p in FOXD2-AS1. (B, C) Relative expression levels of FOXD2-AS1 and miR-185-5p in U87 and U251 cells were detected by RIP assay. (D) U87 and U251 cells were co-transfected with FOXD2-AS1-wt (or FOXD2-AS1-mut) and miR-185-5p (or miR-control) and the luciferase activity was determined by the luciferase reporter assay. Control, non-transfected cells; NC, cells transfected with miR-control. **p < 0.01 vs. Control; ##p < 0.01 vs. NC. (E) Relative expression of FOXD2-AS1 after transfection with FOXD2-AS1 siRNA (si-FOXD2-AS1) in U251 and U87 cells was detected qRT-PCR. Control, non-transfected cells; si-NC, siRNA-control. **p < 0.01 vs. Control; ##p < 0.01 vs. si-NC. (F) Relative expression of miR-185-5p after knockdown of FOXD2-AS1 in U251 and U87 cells was detected qRT-PCR. Control, non-transfected cells; si-NC, siRNA-control. **p < 0.01 vs. Control; ##p < 0.01 vs. si-NC. (G) Relative expression of miR-185-5p after transfection with miR-185-5p inhibitor in U251 and U87 cells was detected qRT-PCR. Control, non-transfected cells; si-NC, siRNA-control. **p < 0.01 vs. Control; ##p < 0.01 vs. si-NC. (H) Relative expression of FOXD2-AS1 after inhibition of miR-185-5p in U251 and U87 cells was detected qRT-PCR. Control, non-transfected cells; NC, cells transfected with miR-control.