Figure 4. MiR-185-5p targets HMGA2. (A) The binding site of miR-185-5p in the 3’UTR of HMGA2 was predicted via TargetScan. (B) Correlation between HMGA2 and miR-185-5p was validated in a luciferase reporter assay. (C, D) Protein and mRNA expression of HMGA2 after transfection with miR-185-5p mimic in U251 cells were analyzed by Western blot and qRT-PCR, respectively. (E-H) Effects of the FOXD2-AS1/miR-185-5p axis on HMGA2 protein (E, F) and mRNA (G, H) expression were analyzed by Western blot and qRT-PCR, respectively. **p < 0.01 vs. Control; ##p < 0.01 vs. NC; &&p < 0.01 vs. si-FOXD2-AS1.