Research Paper Volume 11, Issue 5 pp 1427—1439

FoxD2-AS1 promotes glioma progression by regulating miR-185-5P/HMGA2 axis and PI3K/AKT signaling pathway

Figure 5. FOXD2-AS1 regulates the proliferation and migration of glioma cells by miR-185-5p/HMGA2-mediated AKT signaling. (A, B) The influence of miR-185-5p/HMGA2 axis on the proliferation of U251 and U87 cells was analyzed by MTT. (C,D) The effect of miR-185-5p/HMGA2 axis on the migration ability of U251 and U87 cells was analyzed by Transwell migration assay (200×). (E, F) Overexpression of HMGA2 abolished the inhibitory effects of FOXD2-AS1 on cell growth and migration in U251 cells as determined by MTT and migration assay. (G) Effects of the FOXD2-AS1/miR-185-5p/HMGA2 on the mRNA level of PI3K. (H) Effects of the FOXD2-AS1/miR-185-5p/HMGA2 on the levels of PI3K and phospho-Akt (p-AKT) in U251 and U87 cells were examined by Western blot. (I, J) Treatment of SC79 blocked the suppressive effects of FOXD2-AS1 on cell growth and migration in U251 cells. MTT assay and transwell assay were performed with transfected cells that treated with SC79 (5 µg/ml) for 48 h (I) and 24 h (J), respectively. **p < 0.01 vs. Control; ##p < 0.01 vs. NC; &&p < 0.01 vs si-FOXD2-AS1.