Research Paper Volume 11, Issue 5 pp 1456—1470

Long intergenic non-coding LINC00657 regulates tumorigenesis of glioblastoma by acting as a molecular sponge of miR-190a-3p

Figure 4. Overexpression of LINC00657 inhibited viability and colony formation in GBM cells via enhancing cell apoptosis. Cell viability of LN-18 (A) and U-118MG (B) after transfecting with pcDNA3.1-NC or pcDNA3.1-LINC00657 for 48 h was detected with CCK-8 assay. Values were represented as mean ± SD. **P<0.01, compared with blank group, ANOVA analysis. (C) Cell colony formation stained with crystal violet of LN-18 and U-118MG after transfecting with pcDNA3.1-NC or pcDNA3.1-LINC00657. (D) The calculated value of stained cells in cell colony formation of LN-18 and U-118MG after transfecting with pcDNA3.1-NC or pcDNA3.1-LINC00657. (E) Cell apoptosis of LN-18 and U-118MG after transfecting with pcDNA3.1-NC or pcDNA3.1-LINC00657 was detected with flow cytometry. (F) Apoptosis rate of LN-18 and U-118MG after transfecting with pcDNA3.1-NC or pcDNA3.1-LINC00657. (G) LN-18 and U-118MG proliferation after transfecting with pcDNA3.1-NC or pcDNA3.1-LINC00657 using EdU and DAPI staining. (H) EdU positive cell rate of LN-18 and U-118MG after transfecting with pcDNA3.1-NC or pcDNA3.1-LINC00657. At least 3 randomly observed fields were chosen to calculate the rate in each group. **P<0.01, compared with control group, unpaired t-tests.