Research Paper Volume 11, Issue 5 pp 1456—1470

Long intergenic non-coding LINC00657 regulates tumorigenesis of glioblastoma by acting as a molecular sponge of miR-190a-3p

Figure 8. Inhibitory effects of LINC00657 on GBM tumor growth was reversed by VO-Ohpic. (A) Cell viability of LN-18 and U-118MG after transfecting with pcDNA3.1-LINC00657 with or without VO-Ohpic (100 nM) for 48 h was detected CCK-8 assay. (B) Cell apoptosis of LN-18 and U-118MG was detected with flow cytometry. (C) Apoptosis rate of LN-18 and U-118MG. (D) The protein level of p-Akt in GBM cells after transfecting with pcDNA3.1-LINC00657 with or without VO-Ohpic for 48 h was measured with western blot. (E) Relative p-Akt levels in the cells. **P<0.01.