Research Paper Volume 11, Issue 5 pp 1471—1485

LINC00702 suppresses proliferation and invasion in non-small cell lung cancer through regulating miR-510/PTEN axis

Figure 3. Overexpression of LINC00702 inhibited NSCLC cell proliferation and invasion via inducing apoptosis in vitro. (A) Quantitative RT-PCR analysis of relative LINC00702 expression levels in NCI-H441 and PC-9 transfected with pcDNA3.1, pcDNA3.1-LINC00702 or NC. (B, C) CCK-8 assays were used to evaluate the effect of LINC00702 on proliferation ability of NCI-H441 and PC-9 cells. NCI-H441 and PC-9 cells were transfected with pcDNA3.1 or pcDNA3.1-LINC00702 for 72 h and subjected to CCK-8 assays (D) EdU staining was performed in NCI-H441 and PC-9 cells. (E, F) Colony formation assays were used to evaluate the effect of LINC00702 on anchor-independent growth ability of NCI-H441 and PC-9 cells. NCI-H441 and PC-9 cells were transfected with pcDNA3.1 or pcDNA3.1-LINC00702 for 72 h and subjected to colony formation assays. (G, H). NCI-H441 and PC-9 cells were transfected with pcDNA3.1 or pcDNA3.1-LINC00702 for 72 h and subjected to transwell assay. (I, J) Flow cytometry was used to evaluate the effect of LINC00702 on apoptosis of NCI-H441 and PC-9 cells. **P<0.01 was considered to indicate a statistically significant difference.