Research Paper Volume 11, Issue 5 pp 1471—1485

LINC00702 suppresses proliferation and invasion in non-small cell lung cancer through regulating miR-510/PTEN axis

Figure 4. LINC00702 functioned as a ceRNA for miR-510 in NSCLC cells. (A) Quantitative RT-PCR analysis of relative miRNAs expression levels in NCI-H441 cells transfected with pcDNA3.1 or pcDNA3.1-LINC00702. (B) Quantitative RT-PCR analysis of relative miRNAs expression levels in PC-9 cells transfected with pcDNA3.1 or pcDNA3.1-LINC00702. (C) Quantitative RT-PCR analysis of relative miRNA-510 expression levels in 40 pairs NSCLC tumor and adjacent tissues. P<0.01, two-way ANOVA. (D) Pearson's correlation scatter plot of the fold change of LINC00702 and miR-510 in NSCLC tumor tissues. (E) The predicted binding sites in LINC00702 and miR-510 and the mutant sequence of LINC00702. (F, G) A firefly luciferase reporter containing either wild-type (WT) or mutant (MT) LINC00702 was transfected with miR-510 mimics in NCI-H441 or PC-9 cells. (H) LINC00702 pulled down by biotinylated miR-510 in the NSCLC cells was detected by qRT-PCR. **P<0.01 was considered to indicate a statistically significant difference.