Research Paper Volume 11, Issue 5 pp 1471—1485

LINC00702 suppresses proliferation and invasion in non-small cell lung cancer through regulating miR-510/PTEN axis

Figure 5. MiR-510 directly targeted PTEN gene. (A) The predicted binding sites in PTEN and miR-510 and the mutant sequence of PTEN. (B) A firefly luciferase reporter containing either wild-type (WT) or mutant (MT) PTEN was transfected with miR-510 mimics (20 nM) in NCI-H441 cells. (C) A firefly luciferase reporter containing either wild-type (WT) or mutant (MT) PTEN was transfected miR-510 mimics (20 nM) in PC-9 cells. (D) Quantitative RT-PCR analysis of relative PTEN expression levels in NSCLC cells transfected with control mimics or miR-510 mimics. (E) Quantitative RT-PCR analysis of relative PTEN expression levels in NSCLC cells transfected with pcDNA3.1-LINC00702 or/and miR-510 mimics. *P<0.05 and **P<0.01 were considered to indicate a statistically significant difference.