Research Paper Volume 11, Issue 6 pp 1695—1715

Long non-coding RNA CDKN2B-AS1 reduces inflammatory response and promotes cholesterol efflux in atherosclerosis by inhibiting ADAM10 expression

Figure 2. CDKN2B-AS1 suppresses atherosclerotic inflammatory response and promotes cholesterol efflux. (A) the infection efficiency of lentiviral vector expressing oe-CDKN2B-AS1; (B) liquid scintillation counter was used to detect the cholesterol efflux of each group; (C) oil red O staining was used to detect the intracellular lipid accumulation of each group (× 200), the red arrows indicate intracellular lipid particles after staining; (D) ELISA was used to detect the levels of IL-1β and TNF-ɑ in each group; * p < 0.05 vs. the oe-NC group; the measurement data were expressed in the form of mean ± standard deviation and analyzed by unpaired t-test, the experiment was repeated 3 times; NC, negative control; ELISA, enzyme linked immunosorbent assay; TNF, tumor necrosis factor; IL, interleukin; CDKN, cell-dependent kinase inhibitor.