Research Paper Volume 11, Issue 6 pp 1695—1715

Long non-coding RNA CDKN2B-AS1 reduces inflammatory response and promotes cholesterol efflux in atherosclerosis by inhibiting ADAM10 expression

Figure 4. ADAM10 silencing suppresses inflammatory response and promotes cholesterol efflux in atherosclerosis. (A) the expression of ADAM10 after infection with lentiviral vector expressing sh-ADAM10 determined by RT-qPCR; (B) liquid scintillation counter was used to detect the cholesterol efflux of cells infected with lentiviral vector expressing sh-ADAM10; (C) oil red O staining was used to detect the lipid accumulation of cells infected with lentiviral vector expressing sh-ADAM10 (× 200), the red arrows indicate intracellular lipid particles after staining; (D) ELISA was used to detect IL-1β and TNF-ɑ in cells infected with lentiviral vector expressing sh-ADAM10; * p < 0.05 vs. the sh-NC group; the measurement data were expressed in the form of mean ± standard deviation and analyzed by unpaired t-test, the experiment was repeated 3 times; ELISA, enzyme linked immunosorbent assay; TNF, tumor necrosis factor; IL, interleukin.